Research
Thus far, I have participated in three independent laboratories that span the range of epigenetics, genetics, and cardiac related projects.
While at Pitzer College, I joined Dr. Emily Wiley's laboratory and conducted novel epigenetic research on the histone deacetylase Thd2 in Tetrahymena thermophila. Specifically, I examined Thd2’s effects on histone acetylation and methylation in both euchromatin and heterochromatin. The findings of this research formulated my thesis in molecular biology and were presented to the faculty and students of The Claremont Colleges.
Immediately after receiving my bachelor's degree, I traveled to Tübingen, Germany to further my research experience at the Friedrich Miescher Laboratory of the Max Planck Society. I joined Dr. Felicity Jones' laboratory to study the molecular basis of adaptive divergence and speciation in natural vertebrate populations using three-spined stickleback fish as a model organism. While at the Max Planck Institute, I helped design experiments to discover recombination hotspots using microsatellites and DNA sequencing, while also using molecular cloning techniques to create plasmids for transfection.
After studying the genetics of speciation, I joined Dr. Joseph Woo laboratory at Stanford’s Cardiothoracic Surgery Department. The multidisciplinary nature of the Woo lab offered countless opportunities to expand my research knowledge. I contributed to a broad range of cardiac research projects including RNA-sequencing studies of human cardiac tissue, utilizing cyanobacteria to re-oxygenate ischemic tissue, engineering and utilizing an ex vivo heart pump to measure pressures and flow rates pre and post cardiac operations, developing synthetic blood vessels, and delivering bioengineered, therapeutic cytokines into cardiac tissue in an ovine myocardial infarction model via a sheer-thinning hydrogel, among several others. These efforts amounted to several publications.
Publications
Jaatinen, K., Shah, P., Mazhari, R., Hayden, Z., Wargowsky, R., Jepson, T., Toma, I., Perkins, J., & McCaffrey, T. A. (2024). RNAseq of INOCA patients identifies innate, invariant, and acquired immune changes: potential autoimmune microvascular dysfunction. Frontiers in cardiovascular medicine, 11, 1385457.
Cruz, P. D., Wargowsky, R., Gonzalez-Almada, A., Sifontes, E. P., Shaykhinurov, E., Jaatinen, K., Jepson, T., Lafleur, J. E., Yamane, D., Perkins, J., Pasquale, M., Giang, B., McHarg, M., Falk, Z., & McCaffrey, T. A. (2024). Blood RNA Biomarkers Identify Bacterial and Biofilm Coinfections in COVID-19 Intensive Care Patients. Journal of intensive care medicine, 8850666241251743. Advance online publication. https://doi.org/10.1177/08850666241251743.
Traeger, G.R., Jaatinen, K.J., Majesky, M.W., & Greene C.L (2023). The Advent of Spatial Omics in Congenital Heart Disease. Curr Treat Options Peds. https://doi.org/10.1007/s40746-023-00282-1.
McCaffrey, T. A., Toma, I., Yang, Z., Katz, R., Reiner, J., Mazhari, R., Shah, P., Falk, Z., Wargowsky, R., Goldman, J., Jones, D., Shtokalo, D., Antonets, D., Jepson, T., Fetisova, A., Jaatinen, K., Ree, N., & Ri, M. (2023). RNAseq profiling of blood from patients with coronary artery disease: Signature of a T cell imbalance. Journal of molecular and cellular cardiology plus, 4, 100033. https://doi.org/10.1016/j.jmccpl.2023.100033.
Shin, H. S., Thakore, A., Tada, Y., Pedroza, A. J., Ikeda, G., Chen, I. Y., Chan, D., Jaatinen, K. J., Yajima, S., Pfrender, E. M., Kawamura, M., Yang, P. C., Wu, J. C., Appel, E. A., Fischbein, M. P., Woo, Y., & Shudo, Y. (2022). Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling. Scientific reports, 12(1), 17605.
Williams KM, Wang H, Paulsen MJ, Thakore AD, Rieck M, Lucian HJ, Grady F, Hironaka CE, Chien AJ, Farry JM, Shin HS, Jaatinen KJ, Eskandari A, Stapleton LM, Steele AN, Cohen JE, Woo YJ. Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria-mammalian symbiotic therapeutics. Microb Biotechnol. 2020 Nov;13(6):1780-1792. doi: 10.1111/1751-7915.13596. Epub 2020 May 31. PubMed PMID: 32476224; PubMed Central PMCID: PMC7533327.
Greene CL, Jaatinen KJ, Wang H, Koyano TK, Bilbao MS, Woo YJ. Transcriptional Profiling of Normal, Stenotic, and Regurgitant Human Aortic Valves. Genes (Basel). 2020 Jul 14;11(7). doi: 10.3390/genes11070789. PubMed PMID: 32674273; PubMed Central PMCID: PMC7397246.
Shudo Y, MacArthur JW, Kunitomi Y, Joubert L, Kawamura M, Ono J, Thakore A, Jaatinen K, Eskandari A, Hironaka C, Shin HS, Woo YJ. Three-Dimensional Multilayered Microstructure Using Needle Array Bioprinting System. Tissue Eng Part A. 2020 Mar;26(5-6):350-357. doi: 10.1089/ten.TEA.2019.0313. PubMed PMID: 32085692; PubMed Central PMCID: PMC7476375.
Steele AN, Paulsen MJ, Wang H, Stapleton LM, Lucian HJ, Eskandari A, Hironaka CE, Farry JM, Baker SW, Thakore AD, Jaatinen KJ, Tada Y, Hollander MJ, Williams KM, Seymour AJ, Totherow KP, Yu AC, Cochran JR, Appel EA, Woo YJ. Multi-phase catheter-injectable hydrogel enables dual-stage protein-engineered cytokine release to mitigate adverse left ventricular remodeling following myocardial infarction in a small animal model and a large animal model. Cytokine. 2020 Mar;127:154974. doi: 10.1016/j.cyto.2019.154974. Epub 2020 Jan 21. PubMed PMID: 31978642.
Pedroza AJ, Koyano T, Trojan J, Rubin A, Palmon I, Jaatinen K, Burdon G, Chang P, Tashima Y, Cui JZ, Berry G, Iosef C, Fischbein MP. Divergent effects of canonical and non-canonical TGF-β signalling on mixed contractile-synthetic smooth muscle cell phenotype in human Marfan syndrome aortic root aneurysms. J Cell Mol Med. 2020 Feb;24(3):2369-2383. doi: 10.1111/jcmm.14921. Epub 2019 Dec 30. PubMed PMID: 31886938; PubMed Central PMCID: PMC7011150.
Stapleton LM, Steele AN, Wang H, Lopez Hernandez H, Yu AC, Paulsen MJ, Smith AAA, Roth GA, Thakore AD, Lucian HJ, Totherow KP, Baker SW, Tada Y, Farry JM, Eskandari A, Hironaka CE, Jaatinen KJ, Williams KM, Bergamasco H, Marschel C, Chadwick B, Grady F, Ma M, Appel EA, Woo YJ. Use of a supramolecular polymeric hydrogel as an effective post-operative pericardial adhesion barrier. Nat Biomed Eng. 2019 Aug;3(8):611-620. doi: 10.1038/s41551-019-0442-z. Epub 2019 Aug 7. PubMed PMID: 31391596.
von Bornstädt D, Wang H, Paulsen MJ, Goldstone AB, Eskandari A, Thakore A, Stapleton L, Steele AN, Truong VN, Jaatinen K, Hironaka C, Woo YJ. Rapid Self-Assembly of Bioengineered Cardiovascular Bypass Grafts From Scaffold-Stabilized, Tubular Bilevel Cell Sheets. Circulation. 2018 Nov 6;138(19):2130-2144. doi: 10.1161/CIRCULATIONAHA.118.035231. PubMed PMID: 30474423; PubMed Central PMCID: PMC6261325.
Kawamura M, Paulsen MJ, Goldstone AB, Shudo Y, Wang H, Steele AN, Stapleton LM, Edwards BB, Eskandari A, Truong VN, Jaatinen KJ, Ingason AB, Miyagawa S, Sawa Y, Woo YJ. Tissue-engineered smooth muscle cell and endothelial progenitor cell bi-level cell sheets prevent progression of cardiac dysfunction, microvascular dysfunction, and interstitial fibrosis in a rodent model of type 1 diabetes-induced cardiomyopathy. Cardiovasc Diabetol. 2017 Nov 2;16(1):142. doi: 10.1186/s12933-017-0625-4. PubMed PMID: 29096622; PubMed Central PMCID: PMC5668999.
Cohen JE, Goldstone AB, Paulsen MJ, Shudo Y, Steele AN, Edwards BB, Patel JB, MacArthur JW Jr, Hopkins MS, Burnett CE, Jaatinen KJ, Thakore AD, Farry JM, Truong VN, Bourdillon AT, Stapleton LM, Eskandari A, Fairman AS, Hiesinger W, Esipova TV, Patrick WL, Ji K, Shizuru JA, Woo YJ. An innovative biologic system for photon-powered myocardium in the ischemic heart. Sci Adv. 2017 Jun;3(6):e1603078. doi: 10.1126/sciadv.1603078. eCollection 2017 Jun. PubMed PMID: 28630913; PubMed Central PMCID: PMC5470824.
Presentations
Jaatinen, K.J. (2023, Nov 15). Sequencing of blood RNA from INOCA patients identifies changes in innate, invariant, and acquired immune pathways. [Online Grand Rounds]. GW Cardiology Department.
Jaatinen, K.J. (2024, April 7). Sequencing of blood RNA from INOCA patients identifies changes in innate, invariant, and acquired immune pathways. [Poster presentation]. ACC.24, Atlanta, GA.
Jaatinen, K.J. (2024, April 25). Sequencing of blood RNA from INOCA patients identifies changes in innate, invariant, and acquired immune pathways. [Poster presentation]. GW Research Day, Washington, DC.